maladorit
发表于 2025-3-25 03:56:29
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2否定
发表于 2025-3-25 08:33:34
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到婚嫁年龄
发表于 2025-3-25 13:02:31
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使虚弱
发表于 2025-3-25 19:49:06
Book 2019 targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and dete
BATE
发表于 2025-3-25 21:59:12
Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine, aspects need to be considered before starting such a project. Below we describe a detailed step-by-step protocol for planning and performing a transcriptome-wide bisulfite sequencing experiment and subsequent data analysis to determine methyl-cytosine in poly(A)RNA from cells and tissues.
得意牛
发表于 2025-3-26 00:38:53
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人充满活力
发表于 2025-3-26 07:13:14
,Direct Chemical Biotinylation of RNA 5′-Ends Using a Diazo Reagent,n-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditions (~pH 6.8), the 5′-phosphate carries the only mildly acidic proton in the RNA molecule, which allows for selective functionalization at that site using diazo reagents.
Subjugate
发表于 2025-3-26 09:03:24
Identification of Methylated Transcripts Using the TRIBE Approach,ion. Here, we describe a complementary technique of standard meRIP-seq/miCLIP-seq approaches to identify methylated RNA using a low amount of material. We believe this approach can be applied in vivo to identify methylated targets in specific tissues or subpopulations of cells.
GOAD
发表于 2025-3-26 16:23:49
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Foolproof
发表于 2025-3-26 20:16:57
Peter Kopietz,Lorenz Bartosch,Florian SchützPR/Cas9-based approach. This strategy is relatively simple approach to track . at different stages of cell differentiation, providing mechanistic insights into the initiation, maintenance, and establishment of X inactivation.