hurricane
发表于 2025-3-27 00:42:19
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值得尊敬
发表于 2025-3-27 01:39:06
Is Caste Already Part of UK Equality Law?,in imaging and diagnostics. Here we describe the modification of proteins using TTL in detail via a one-step as well as two-step procedure and highlight its practicability for applications in imaging, diagnostics, and cell biology.
insurrection
发表于 2025-3-27 06:00:55
Butelase 1-Mediated Ligation of Peptides and Proteins,abeling, preparation of thioesters, and bioconjugation of dendrimers. Additionally, we provide an example using butelase 1 for protein cyclization in combination with genetic code expansion in order to incorporate unnatural building blocks.
Excise
发表于 2025-3-27 10:57:04
SpyLigase-Catalyzed Modification of Antibodies,rom . cells, chemical solid-phase synthesis of the KTag peptide and its coupling to reporter molecules and cytotoxins as well as the transient expression from mammalian cells to produce Spy-tagged antibodies. Furthermore, we describe the purification and analytics of the formed conjugates.
CLASH
发表于 2025-3-27 14:24:35
Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation, handle that is then used for drug molecule attachment in a second step. The aim of this chapter is to outline detailed protocols for both labeling procedures, as well as to provide guidance on enzyme and substrate preparation.
STIT
发表于 2025-3-27 21:00:25
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重力
发表于 2025-3-28 01:02:27
Book 2019to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial tra
fabricate
发表于 2025-3-28 02:58:11
1064-3745 ation advice from the experts.This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as s
overweight
发表于 2025-3-28 06:27:18
After-Sales-Feedback mit Kundenkonferenzenaction conditions. In this chapter, we describe proximity-based sortase-mediated ligation (PBSL), which uses the SpyTag–SpyCatcher peptide–protein pair to link SrtA to target proteins and dramatically improves reaction rate and ligation efficiency.
极大痛苦
发表于 2025-3-28 10:55:15
https://doi.org/10.1007/978-981-10-7092-1 .terminal NMT recognition sequence are covalently modified with an azido fatty acid. Subsequent strain-promoted azide–alkyne cycloaddition allows for conjugation to cell-permeant fluorophores and imaging by fluorescence microscopy. Here we describe sample preparation and labeling protocols for imaging bacterial proteins in fixed and live cells.