夸张 发表于 2025-3-25 05:44:23

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Paradox 发表于 2025-3-25 08:42:52

Chemoenzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides Using Omniligase-1, we provide protocols for the chemoenzymatic synthesis of the peptide therapeutic exenatide, a GLP-1 (glucagon-like peptide) analogue, and the macrocyclization and oxidative folding of the cyclotide MCoTI-II in a one-pot procedure.

NAIVE 发表于 2025-3-25 12:25:46

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不感兴趣 发表于 2025-3-25 15:56:50

BioID as a Tool for Protein-Proximity Labeling in Living Cells,d transient interactions within a relevant biological setting over a defined period of time. Here we briefly review the scientific progress enabled by the BioID technology, detail an updated protocol for applying the method to proteins in living cells, and offer insights for troubleshooting commonly encountered setbacks.

高脚酒杯 发表于 2025-3-25 21:28:01

Cyclizing Disulfide-Rich Peptides Using Sortase A, application of SrtA as a tool for the cyclization of disulfide-rich peptides. Reactions are typically tailored to each disulfide-rich peptide with optimal conditions producing yields of 40–50% cyclized peptide.

Immortal 发表于 2025-3-26 03:21:28

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Inflated 发表于 2025-3-26 08:15:01

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foppish 发表于 2025-3-26 08:56:39

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BATE 发表于 2025-3-26 15:50:49

https://doi.org/10.1007/978-3-319-59132-2abeling, preparation of thioesters, and bioconjugation of dendrimers. Additionally, we provide an example using butelase 1 for protein cyclization in combination with genetic code expansion in order to incorporate unnatural building blocks.

分贝 发表于 2025-3-26 19:47:30

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查看完整版本: Titlebook: Enzyme-Mediated Ligation Methods; Timo Nuijens,Marcel Schmidt Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019