DAUNT
发表于 2025-3-26 22:30:12
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Coordinate
发表于 2025-3-27 03:22:04
Wolfgang H. Runde,Wallace W. SchulzPNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pat
bourgeois
发表于 2025-3-27 06:34:08
https://doi.org/10.1007/978-1-0716-2273-5Bioactive metabolites; Drug discovery; Transcriptomes; Bioengineering; Non-natural natural products
oncologist
发表于 2025-3-27 11:51:56
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Tracheotomy
发表于 2025-3-27 13:36:55
Adducts and Heterometallic Alkoxides,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.
起波澜
发表于 2025-3-27 19:16:58
Sediment and the phosphate cycle,we report a standard experimental protocol for the deletion of a biosynthetic gene in a . species, using the vector pCRISPomyces-2 developed by Huimin Zhao and collaborators. We also describe how carrying out metabolite analysis can reveal the putative biosynthetic function of the inactivated gene.
抛媚眼
发表于 2025-3-27 22:52:31
Targeted Genetic Engineering via ,-Mediated Transformation in ,box is missing for the investigation of gene function and metabolism. In this chapter, we describe how .-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in ., is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in . f. sp. ..
湿润
发表于 2025-3-28 02:42:53
Investigating Fungal Biosynthetic Pathways Using , as a Heterologous Host,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal biosynthetic pathways.
Kindle
发表于 2025-3-28 07:59:15
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chisel
发表于 2025-3-28 13:47:23
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