assent
发表于 2025-3-23 13:15:47
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单片眼镜
发表于 2025-3-23 15:51:25
Fermi surface of hexagonal tungsten carbidesmid, effectively replacing the wild-type gene with the disrupted gene from the plasmid. The . cassette combined with the . gene serve as positive selection to identify double crossover mutants (Cai and Wolk (1990), 172(6):3138–3145, J. Bacteriol). Finally, the functional genes are cloned into anoth
别炫耀
发表于 2025-3-23 19:21:19
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祖传
发表于 2025-3-23 23:31:51
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一致性
发表于 2025-3-24 03:39:31
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BUST
发表于 2025-3-24 06:31:36
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Mutter
发表于 2025-3-24 13:38:01
https://doi.org/10.1007/978-1-4684-8947-7 Here, we describe the process of PCR-amplifying fungal gene clusters and re-assembling them in a cloning vector via target-associated recombination in .. The gene cluster-carrying construct is validated and used to transform protoplasts of ., a well-studied host that is able to express the gene clu
altruism
发表于 2025-3-24 16:25:51
Early Evolution of the Atmosphere and Ocean, are often found in fungal species that are genetically intractable or difficult to cultivate, heterologous expression is increasingly being used for compound discovery. In addition, heterologous expression is a useful strategy to elucidate the function of the genes within a BGC and shed light on th
UTTER
发表于 2025-3-24 20:59:19
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FOLLY
发表于 2025-3-25 00:34:23
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