绅士
发表于 2025-3-30 10:22:11
https://doi.org/10.1007/978-3-322-95197-7S based on good manufacturing practice and cell therapy-grade reagents. We further describe fully defined protocols to terminally differentiate lt-NES toward GABA-ergic, dopaminergic, and motor neurons.
防锈
发表于 2025-3-30 14:25:06
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改变立场
发表于 2025-3-30 17:03:32
https://doi.org/10.1007/978-1-4302-0848-8cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.
网络添麻烦
发表于 2025-3-30 21:56:25
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tenosynovitis
发表于 2025-3-31 02:29:17
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战役
发表于 2025-3-31 07:01:41
https://doi.org/10.1007/978-3-8349-3650-9quires standard molecular biology techniques and lab equipment. It allows for accurate 3′ counting of transcript at the single-cell level, which helps reveal cellular identities during EB formation. Combined with perturbation experiments, the method provides an opportunity for mechanistic studies of embryo development at the single-cell level.
小丑
发表于 2025-3-31 10:57:07
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天空
发表于 2025-3-31 15:51:04
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FEAS
发表于 2025-3-31 17:55:19
Feeder-Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol,lished for mESCs. Here we describe the isolation, culture, and preparation feeder cell layers and establishment of feeder-dependent/independent protocol for mESC culture. In addition, basic mESC protocols for culture, storage, and differentiation were described.
障碍
发表于 2025-4-1 01:01:04
,Replating Protocol for Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes,cols to dissociate hiPSC-CMs by using collagenase A&B, Collagenase II, TrypLE, and EDTA and reseeding on various matrix materials including fibronectin, laminin, imatrix, Matrigel, and Geltrex. By the replating methods described here, a single cell or cluster-containing hiPSC-CM cultures can be generated effectively.