cajole
发表于 2025-3-23 09:50:09
https://doi.org/10.1007/978-3-476-03665-0 powerful tool to investigate the mechanisms underlying the pluripotent state transition. Here, we describe a defined and robust protocol to transiently induce mEpiLCs from mESCs, together with a concise overview for their unbiased characterization for subsequent downstream applications.
仔细检查
发表于 2025-3-23 17:24:25
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Ordnance
发表于 2025-3-23 19:07:49
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N防腐剂
发表于 2025-3-23 23:42:32
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Ovulation
发表于 2025-3-24 02:27:12
Anthony A. Peguero,Jun Sung Hongntiation method to generate inner ear organoids containing sensory epithelia with hair cells. Human pluripotent stem cells are aggregated in low-binding 96-well plates and treated in chemically defined media with extracellular matrix to promote epithelialization. Small molecules and recombinant prot
漫步
发表于 2025-3-24 06:33:09
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defeatist
发表于 2025-3-24 14:45:12
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松紧带
发表于 2025-3-24 16:10:10
https://doi.org/10.1007/978-1-84628-660-5used to isolate and quantitatively characterize human and mouse hematopoietic cells, often using fluorescently labeled antibodies. However, such analysis is limited in zebrafish due to lack of antibodies that recognize zebrafish hematopoietic cells. We here describe methods for isolation of hematopo
一回合
发表于 2025-3-24 20:12:42
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Gentry
发表于 2025-3-25 01:50:10
https://doi.org/10.1007/978-981-10-7467-7derm, and endoderm. Pluripotency is usually demonstrated in vitro by spontaneous differentiation of hESCs grown on a monolayer of feeder-cells using an embryoid bodies (EBs)-based method. However, currently hESCs are grown mostly using fully defined media in the absence of a feeder layer. Here we de