nonsensical 发表于 2025-3-26 22:38:11

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Hippocampus 发表于 2025-3-27 02:04:22

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pacifist 发表于 2025-3-27 07:40:28

https://doi.org/10.1007/978-3-031-08333-4ert for digestion and to protect the primer site. The “Cyclone Sequencing” technique of Dale, McClare, and Houchins (.) overcomes this problem as it is totally independent of the restriction sites in the insert. In this procedure single-stranded Ml3 is hybridized to an oligomer that spans the .dIII

使苦恼 发表于 2025-3-27 09:34:40

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多骨 发表于 2025-3-27 13:52:20

K. Bennaceur,Z. Sahraoui,M. A. Nacerherefore, it became the method of choice to obtain several hundred bases of sequence information per reaction. The procedure is based on the enzymatic elongation and radioactive labeling of oligonucleotides that are complementary to the beginning of the single-stranded DNA template. Chain extension

curriculum 发表于 2025-3-27 17:46:10

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训诫 发表于 2025-3-28 01:14:55

Sesan Akintade,Seongtae Kim,Kaushik Royng a DNA polymerase (.). Several different enzymes are available for this purpose each having different qualities and properties. The use of the Klenow fragment of DNA polymerase I, DNA polymerase, and T7 DNA polymerase in DNA sequence analysis is described elsewhere in this volume.

WAX 发表于 2025-3-28 06:02:51

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看法等 发表于 2025-3-28 07:32:32

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figment 发表于 2025-3-28 13:19:17

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查看完整版本: Titlebook: DNA Sequencing Protocols; Hugh G. Griffin,Annette M. Griffin Book 19931st edition Springer Science+Business Media New York 1993