强壮 发表于 2025-4-1 02:00:01
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http://reply.papertrans.cn/27/2602/260196/260196_62.pngHUMP 发表于 2025-4-1 10:27:33
Subcloning for DNA Sequencing, are very inefficient at completing the last few small sequence gaps that remain near the end of the project. Various nonrandom subcloning methods have also been developed, utilizing partial restriction digests (.), BAL-3 1 digestion (.), exonuclease III digestion (l0–13), and DNase I (.).