从未迷惑
发表于 2025-3-21 19:55:35
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infelicitous
发表于 2025-3-21 21:51:19
Resolving Roadblocks to Telomere Replicationon. The terminal position, repetitive sequence, and structural complexities of the telomeric DNA make the telomere an inherently difficult region to replicate within the genome. Thus, despite functioning to protect genome stability mammalian telomeres are also a source of replication stress and have
江湖郎中
发表于 2025-3-22 01:48:59
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狂怒
发表于 2025-3-22 07:53:24
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micturition
发表于 2025-3-22 09:35:02
Methods to Study Trinucleotide Repeat Instability Induced by DNA Damage and Repaired by DNA replication, repair, recombination, and gene transcription. Somatic TNR instability is involved in the progression of TNR expansion diseases and can be modulated by DNA damage repair and gene transcription. Recent studies from our group and others have shown that DNA base damage and its re
Mangle
发表于 2025-3-22 14:30:28
Extracting and Measuring dNTP Pools in ility. Ribonucleotide reductase (RNR) catalyzes the rate-limiting step in dNTP synthesis and altered regulation of RNR leads to imbalanced dNTP pools. Increased dNTP levels are mutagenic and have the potential to interfere with pathways that are involved in DNA replication, repair and DNA damage con
Mangle
发表于 2025-3-22 17:03:11
Conversion Tract Analysis of Homology-Directed Genome Editing Using Oligonucleotide Donorsnversion tract is defined as the amount of genetic information that is converted from the homology donor to a given strand of the targeted chromosomal locus. Because of this, conversion tract analysis retrospectively not only elucidates the mechanism of homology-directed genome editing but also prov
真繁荣
发表于 2025-3-22 21:36:35
Reporter Assays for BER Pathwayamages induced by free radicals and ionizing radiation. Deregulation or deficiencies in BER mechanisms increase the level of mutations leading to carcinogenesis, and single-strand DNA break formation, which may be converted to double-strand breaks and induce apoptosis. BER deficiency is associated w
服从
发表于 2025-3-23 03:40:07
Methods for Studying DNA Single-Strand Break Repair and Signaling in , Egg ExtractsUnrepaired SSBs compromise DNA replication and transcription programs, leading to genome instability, and have been implicated in many diseases including cancer. In this chapter, we introduce methods to study the ATR-Chk1 DNA damage response (DDR) pathway and DNA repair pathway in response to a site
一致性
发表于 2025-3-23 05:58:12
Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in , Egg Extractsitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and . egg extracts provides a highly versatile and tractable approach to analyze dynamic protein–DNA interactions with great spatial and temporal detail.