生长变吼叫 发表于 2025-3-21 18:22:14

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Mast-Cell 发表于 2025-3-21 22:10:42

Generation of MoClo Standard Parts Using Golden Gate Cloning for amplification, (3) performing PCR amplification, (4) cloning of the fragments using Golden Gate cloning, and finally (5) sequencing of the part. For large basic parts, it is preferable to first clone subparts as intermediate level −1 constructs. These subparts are sequenced individually and are

Affection 发表于 2025-3-22 01:23:43

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beta-carotene 发表于 2025-3-22 05:26:40

Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Sw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential.

cauda-equina 发表于 2025-3-22 11:49:37

BASIC: A Simple and Accurate Modular DNA Assembly Methodenter a subsequent BASIC assembly without the need for reformatting or changes to the workflow. This unique idempotent cloning mechanism allows for the assembly of constructs in multiple, conceptionally simple hierarchical rounds. Combined with its high accuracy and robustness, this makes BASIC a ve

燕麦 发表于 2025-3-22 13:51:00

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燕麦 发表于 2025-3-22 18:42:22

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Hemiplegia 发表于 2025-3-23 00:35:53

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ABOUT 发表于 2025-3-23 03:06:45

Die ökonomische Sicht auf die Arbeitw vector is required in order to assemble constructs for any new destination context, such as a new organism or genomic location. This should facilitate more rapid and convenient development of engineered metabolic pathways for diverse nonmodel organisms in order to exploit their applied potential.

maverick 发表于 2025-3-23 07:01:05

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查看完整版本: Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin