Abrade
发表于 2025-3-28 15:53:40
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Intellectual
发表于 2025-3-28 21:53:26
Orientierung an Gebrauchswertento use, efficient, and allow users to assemble multigene constructs by performing a series of one-pot assembly steps, starting from libraries of cloned and sequenced parts. The efficiency of these systems also facilitates the generation of libraries of construct variants. We describe here a protocol
crease
发表于 2025-3-29 00:31:48
Computer — Mega-Maschine oder Werkzeugpe IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set
碎片
发表于 2025-3-29 03:51:20
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清澈
发表于 2025-3-29 09:13:12
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oxidize
发表于 2025-3-29 11:35:57
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Vertical
发表于 2025-3-29 16:17:03
Die ökonomische Sicht auf die Arbeitbly methods. Firstly, coding sequences are assembled with upstream and downstream sequences via overhangs corresponding to start and stop codons, avoiding unwanted ‘scars’ in assembled constructs at coding sequence boundaries. Scars at these crucial, sensitive locations can affect mRNA structure, ac
人造
发表于 2025-3-29 22:17:29
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GILD
发表于 2025-3-30 01:49:26
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trigger
发表于 2025-3-30 05:01:14
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