极深
发表于 2025-3-25 04:39:01
Wendy K. Silverman,William M. Kurtinesregate chromosomes. Several model systems have been widely used to dissect the molecular and structural mechanisms behind mitotic spindle assembly and function. These include budding and fission yeasts, which are ideal for genetic and molecular approaches, but show limitations in high-resolution liv
证明无罪
发表于 2025-3-25 08:22:35
Clinical Child Psychology Libraryules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution
小溪
发表于 2025-3-25 13:24:58
Panic Spectrum Disorders and Substance Usee highly regulated assembly and constriction of an actomyosin contractile ring, whose function is to pinch the mother cell in two. Research on the contractile ring has particularly focused on the signaling mechanisms that dictate when and where the ring is formed. In vivo studies of ring constrictio
山顶可休息
发表于 2025-3-25 17:27:14
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Panacea
发表于 2025-3-25 22:44:26
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Fracture
发表于 2025-3-26 00:23:18
Optogenetic Control of Microtubule Dynamics,gh-resolution live-cell microscopy in combination with π-EB1 photodissociation. However, these techniques are broadly applicable to other LOV2-based and likely other blue light-sensitive optogenetics. In addition to being a tool to investigate +TIP functions acutely and with subcellular resolution,
一骂死割除
发表于 2025-3-26 08:00:16
Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation ofsure spindle microtubule dynamics using photoactivation of fluorescently tagged tubulin in living cells. This methodology allows the quantitative discrimination of the turnover of specific microtubule populations (e.g., kinetochore vs. non-kinetochore microtubules), as well as determination of micro
Urea508
发表于 2025-3-26 11:54:52
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率直
发表于 2025-3-26 14:15:02
Knocking Out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifical enzymes in parallel to obtain a significant change in a given tubulin modification. Here we describe a method to generate primary cells with combinatorial knockout genotypes using conditional knockout mice. The conditional alleles are converted into knockout in the cultured primary cells by transd
ALB
发表于 2025-3-26 20:41:19
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