来这真柔软 发表于 2025-3-30 10:15:04
Conduct Problems and Anxiety in Children,ortical MreB assemblies in vivo. This chapter describes the methods for visualizing fluorescently tagged MreB proteins in live . cells. We detail how to (1) grow . strains for reproducible TIRF observations, (2) immobilize cells on agarose pads and (3) in CellASIC. microfluidic plates, and (4) acquire TIRF images and time lapses.节约 发表于 2025-3-30 15:01:54
Foundations of Developmental Psychopathologyg center. While a number of different approaches and variations thereof have been reported in the literature, here we aim to keep the methodology as simple as possible and thus accessible to most research laboratories.小歌剧 发表于 2025-3-30 19:46:23
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Overview of Anxiety Management Training made to interact with actin filaments through a tip-tracking complex consisting of microtubule end-binding proteins and an actin-microtubule cytolinker. In addition to the protocols themselves, we discuss the optimization steps required in order to build these more complex in vitro model systems of cytoskeletal interactions.土产 发表于 2025-3-31 06:30:50
Evidence for Anxiety Management Traininging useful tools for the quantitative analysis of protein activity and protein-protein interactions. Here, we describe solution-based Förster resonance energy transfer (FRET) and fluorescence assays that can be used to quantify protein-protein interactions and to characterize protein conformations of MT-associated proteins involved in mitosis.Phonophobia 发表于 2025-3-31 11:48:39
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