弯弯曲曲
发表于 2025-3-28 17:50:29
Well-Differentiated Primary Mammalian Airway Epithelial Cell Culturesincluding coronaviruses. In recent years, there has been an increase in interest toward animal AEC cultures and their application to characterize veterinary viruses with zoonotic potential, as well as studying host–pathogen interactions in animal reservoir host species. In this chapter, we provide a
高兴去去
发表于 2025-3-28 20:17:51
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草率男
发表于 2025-3-29 01:52:05
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聋子
发表于 2025-3-29 06:13:37
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipelinestems, however, present several limitations and are sometimes difficult to establish in a timely manner for (re-)emerging viruses. In this chapter, we present a new universal reverse genetics platform for the assembly and engineering of infectious full-length cDNAs using yeast-based transformation-a
LIMN
发表于 2025-3-29 08:10:55
Proximity Labeling for the Identification of Coronavirus–Host Protein Interactionsf enzyme-catalyzed biotin tags ubiquitously apposed on proteins located in close proximity of the labeling enzyme, followed by affinity purification and identification of biotinylated proteins by mass spectrometry. Here we outline the methods by which the molecular microenvironment of the coronaviru
地名表
发表于 2025-3-29 15:09:41
Using Yeast to Identify Coronavirus–Host Protein Interactionsvarious advantages, but in the context of coronavirus research and the system outlined here, they are simple and easy to work with and can be used at biosafety level 2. The system involves inducible expression of individual viral proteins and identification of detrimental phenotypes in the yeast. Ye
offense
发表于 2025-3-29 18:23:30
https://doi.org/10.1007/978-1-0716-0900-2Coronavirinae sub-family; SARS; Virus propagation; Antiviral therapeutics; Virus-host interactions; Diagn
spinal-stenosis
发表于 2025-3-29 22:37:37
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Medicare
发表于 2025-3-30 03:18:14
Coronaviruses978-1-0716-0900-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
cardiac-arrest
发表于 2025-3-30 04:23:21
https://doi.org/10.1007/978-1-84882-628-1RNA genome, and a unique replication strategy. CoVs cause a variety of diseases in mammals and birds ranging from enteritis in cows and pigs, and upper respiratory tract and kidney disease in chickens to lethal human respiratory infections. Most recently, the novel coronavirus, SARS-CoV-2, which was