蘑菇
发表于 2025-3-25 04:06:44
Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitiombinant IBVs are generated in situ following the transfection of vaccinia virus DNA, containing the modified IBV cDNA, into cells infected with a recombinant fowlpox virus expressing T7 DNA-dependent RNA polymerase.
誓言
发表于 2025-3-25 11:09:15
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipelineno specific equipment and can be performed in a standard laboratory setting. The protocol described can be easily adapted to virtually all known or emerging coronaviruses, such as Middle East respiratory syndrome coronavirus (MERS-CoV).
砍伐
发表于 2025-3-25 14:51:09
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Vulnerary
发表于 2025-3-25 18:01:17
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消音器
发表于 2025-3-25 22:57:39
Sverre Petterssen,James Rodger Fleming approaches using BirA. (BioID), TurboID, and APEX2. These factors represent a molecular signature of coronavirus RTCs and likely contribute to the viral life cycle, thereby constituting attractive targets for the development of antiviral intervention strategies.
reptile
发表于 2025-3-26 03:30:13
Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultureshe cultures for the characterization of veterinary coronavirus. We also describe immunohistochemistry protocols with validated antibodies for the visualization and identification of viral cell tropism in well-differentiated AEC cultures from human, swine, bovine, and feline origin.
仪式
发表于 2025-3-26 06:23:10
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Bureaucracy
发表于 2025-3-26 10:41:20
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其他
发表于 2025-3-26 13:17:02
Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Studyed to ST or LLC-PK cells produce cytopathic effects characterized by rounding, clumping, and detachment of cells. PDCoV replication in cells can be quantifiably monitored by qRT-PCR, immunofluorescence assays, and immune-electron microscopy. Infectious viral titers can be evaluated by using plaque a
精美食品
发表于 2025-3-26 19:03:52
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