essential-fats
发表于 2025-3-28 17:25:24
Clinical Applications of PCR978-1-59259-600-3Series ISSN 1543-1894 Series E-ISSN 1940-6037
青石板
发表于 2025-3-28 21:11:08
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不能平静
发表于 2025-3-28 23:25:41
https://doi.org/10.1007/978-981-10-6271-1tissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).
丧失
发表于 2025-3-29 05:43:41
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Nostalgia
发表于 2025-3-29 08:46:05
Julien Bringer,Hervé Chabanne,Linda Guigaegantly simple but the resulting method is extremely powerful. The adoption of the thermostable . polymerase in 1988 greatly simplifies the process and enables the automatron of PCR (.). Since then a large number of applications have been developed that are based on the basic PCR theme. The versatil
CLIFF
发表于 2025-3-29 12:53:04
Community Knowledge About Security:ty has also given PCR its main weakness, namely, its tendency to produce false-positive results due to exogenous contamination (.,.). Contamination avoidance is therefore the single most important consideration when setting up a PCR laboratory, especially one concerned with generating diagnostic inf
micronutrients
发表于 2025-3-29 15:52:09
Community Knowledge About Security:amplify nucleic acid sequences from archival materials, which in many institutions consist of paraffin-embedded tissue samples (.,.). This ability has allowed the carrying out of large scale retrospective studies of archival materials and has facilitated the use of materials from multiple institutio
懒洋洋
发表于 2025-3-29 19:56:59
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MULTI
发表于 2025-3-30 02:24:25
Lecture Notes in Computer Scienceetecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (.). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insu
易怒
发表于 2025-3-30 05:56:35
https://doi.org/10.1007/978-3-031-61231-2ding even one point mutation. A disadvantage of PCR-SSCP, despite its sensitivity, is the necessity to use radioisotopes. To avoid radioisotopes, silver staining was introduced for band detection (.). In the fluorescent amplification refractory mutation system (ARMS)-SSCP using two different fluores