GRIEF
发表于 2025-3-26 21:02:16
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Projection
发表于 2025-3-27 01:58:15
Long Range PCRThe . polymerase lacks proofreading properties (.) and thus is unable to correct such misincorporations. The higher extension K. value for a misincorporated nucleotide is thought to cause detachment of the . polymerase from template DNA.
外来
发表于 2025-3-27 07:27:23
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陈列
发表于 2025-3-27 09:46:57
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DEI
发表于 2025-3-27 14:56:55
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Nefarious
发表于 2025-3-27 19:39:46
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–DOX
发表于 2025-3-28 01:55:19
Sequencing of PCR ProductsIt is often necessary to obtain DNA sequence information following successful amplification. Although polymerase chain reaction (PCR) products may be cloned into a vector and sequenced at a later stage, it is often preferable to sequence PCR products directly, without subcloning, and it is this approach that is addressed in this chapter.
不怕任性
发表于 2025-3-28 04:41:53
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antedate
发表于 2025-3-28 08:48:06
Amplificationtissue specimens. . hybrrdization (ISH) does permit localization of specific nucleic acid sequences at the individual cell level. In conventional nonisotopic . (IS) detection systems, most protocols do not detect single copy genes, except for those incorporating elaborate sandwich detection techniques as described by Herrington et al. (.).
概观
发表于 2025-3-28 11:54:10
PCR Analysis of CD44 Variants in Tumorshain disulfide bonds. It is essentially composed of a 37-kDa protein core highly glycosylated by .- and .-linked sugars to form an 85–90-kDa product and is sometimes additionally linked to chondroitin sulfate side chains to produce a 180–220 kDa form on sodium dodecyl sulfate (SDS) gels (.,.).