Lipohypertrophy
发表于 2025-3-26 22:10:14
Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifuommunication. Among other techniques, the isolation of nerve terminals has been fundamental to study the biochemistry and the physiology of the nervous system. This chapter describes the isolation and purification of intact synaptos
BOON
发表于 2025-3-27 05:04:06
Probing Endocytosis During the Cell Cycle with Minimal Experimental Perturbation, during infection. Endocytosis activity is known to vary during the cell cycle, in particular during mitosis. Importantly, different experimental conditions can lead to opposite results and conclusions, thereby emphasizing the need for a careful design of protocols. For example, experiments using se
可能性
发表于 2025-3-27 07:10:40
Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis, Since the implementation of this methodology to the field of clathrin-mediated endocytosis (CME), this approach has revolutionized our molecular understanding of clathrin-driven cellular uptake. Conventional live cell microscopy approaches are used to determine the precise functions of specific pro
为敌
发表于 2025-3-27 11:59:38
Identifying Small-Molecule Inhibitors of the Clathrin Terminal Domain,ncluding receptors for nutrients and signaling molecules, as well as synaptic vesicle reformation. Multiple genetic and chemical approaches have been developed to interfere with this process. However, many of these tools do not selectively block CME, for example by targeting components shared with c
陈旧
发表于 2025-3-27 16:52:19
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FEMUR
发表于 2025-3-27 18:22:39
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giggle
发表于 2025-3-27 23:12:14
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Ptosis
发表于 2025-3-28 03:22:01
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Kernel
发表于 2025-3-28 07:35:40
Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysi recruitment of auxilin and Hsc70, an ATP-driven molecular clamp. Here, we describe the preparation of reagents and the single-particle fluorescence microscopy imaging assay in which we visualize directly the Hsc70-driven uncoating of synthetic clathrin coats or clathrin-coated vesicles.
HPA533
发表于 2025-3-28 10:54:47
Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Memembrane phospholipids have key regulatory functions in endocytosis and membrane traffic. I have previously described an in vitro assay based on the isolated, substrate-attached plasma membrane to study the spatial distribution and levels of phosphoinositides, in particular phosphatidylinositol-4,5-b