adhesive
发表于 2025-3-25 06:34:23
https://doi.org/10.1007/978-1-4613-9407-5cribe a methodology to follow adaptor-induced clathrin assembly in real-time using fluorescence microscopy on a facile model membrane assay system of .upported .emb.ane .ubes (SMrT). Results from such assays can be conveniently run through routine image analysis procedures to extract kinetic parameters of the clathrin assembly reaction.
Conspiracy
发表于 2025-3-25 11:18:13
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Generalize
发表于 2025-3-25 14:37:46
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共同时代
发表于 2025-3-25 17:12:22
SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions,cribe a methodology to follow adaptor-induced clathrin assembly in real-time using fluorescence microscopy on a facile model membrane assay system of .upported .emb.ane .ubes (SMrT). Results from such assays can be conveniently run through routine image analysis procedures to extract kinetic parameters of the clathrin assembly reaction.
mastoid-bone
发表于 2025-3-25 21:07:46
Francis A. Gunther,Jane Davies Gunthered by mRNA interference or dominant-negative mutant overexpression affect endocytosis long before cells are being assayed. Here, we describe simple experimental procedures to assay endocytosis along the cell cycle with minimal perturbations.
Hypopnea
发表于 2025-3-26 01:59:19
Glenn C. Miller,Richard G. Zepp,5)P. spatial distribution, and semi-quantification of PI(4,5)P. levels in the plasma membrane using fluorescence microscopy. Depending on the probe selected for lipid detection, this simple assay can be modified to study other plasmalemmal phospholipids and/or proteins.
整洁
发表于 2025-3-26 04:46:39
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Libido
发表于 2025-3-26 09:57:00
Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Mem,5)P. spatial distribution, and semi-quantification of PI(4,5)P. levels in the plasma membrane using fluorescence microscopy. Depending on the probe selected for lipid detection, this simple assay can be modified to study other plasmalemmal phospholipids and/or proteins.
飞镖
发表于 2025-3-26 15:49:47
Annette E. McCann,D. Roy Cullimored in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.
我们的面粉
发表于 2025-3-26 19:43:21
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