Amplify
发表于 2025-3-23 10:33:36
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Crayon
发表于 2025-3-23 14:48:55
Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifundamental to study the biochemistry and the physiology of the nervous system. This chapter describes the isolation and purification of intact synaptosomes from rodent brain tissue that can be used to further characterize synaptic structure and function and to examine the molecular mechanisms of neurotransmission.
trigger
发表于 2025-3-23 19:31:06
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BRAND
发表于 2025-3-23 23:20:47
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Between
发表于 2025-3-24 03:08:44
Cryo-Electron Tomography of the Mammalian Synapse, unstained, fully hydrated cellular structures at molecular resolution. The protocol focuses on purified synaptic terminals (“synaptosomes”), currently the most suitable preparation to analyze mammalian synaptic architecture by cryo-ET.
裹住
发表于 2025-3-24 10:18:45
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精密
发表于 2025-3-24 13:51:55
,Imaging “Hot-Wired” Clathrin-Mediated Endocytosis,d in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.
非秘密
发表于 2025-3-24 15:23:32
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知道
发表于 2025-3-24 19:06:44
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连词
发表于 2025-3-25 01:10:45
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