CLOG 发表于 2025-3-21 18:56:09

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hallow 发表于 2025-3-21 23:24:37

978-1-4939-8521-0Springer Science+Business Media, LLC, part of Springer Nature 2018

Alopecia-Areata 发表于 2025-3-22 04:19:01

Circular RNAs978-1-4939-7562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

有权威 发表于 2025-3-22 06:44:23

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Infirm 发表于 2025-3-22 09:53:04

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发表于 2025-3-22 16:30:49

https://doi.org/10.1007/978-3-642-54508-5t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

发表于 2025-3-22 17:57:37

Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR,plice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.

渗透 发表于 2025-3-23 00:37:07

Nonradioactive Northern Blot of circRNAs,y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.

mortgage 发表于 2025-3-23 04:43:23

Preparation of Circular RNA In Vitro,t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

Affirm 发表于 2025-3-23 07:15:51

Christoph Dieterich,Argyris PapantonisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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查看完整版本: Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer