枪支 发表于 2025-3-25 04:38:41
http://reply.papertrans.cn/23/2267/226632/226632_21.png散布 发表于 2025-3-25 10:35:19
https://doi.org/10.1007/978-3-642-54508-5y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probemetropolitan 发表于 2025-3-25 15:15:39
https://doi.org/10.1007/978-3-642-54508-5f them exhibit low expression levels but some are extremely abundant. Typically, circRNAs are studied by RT-PCR-based assays, but for certain types of analyses this technique is not suitable. Circular RNA with repetitive exons (circular concatemers) has been observed by us and others when transientlincubus 发表于 2025-3-25 17:23:32
https://doi.org/10.1007/978-3-642-54508-5n coding potential, currently classified as long noncoding RNAs (lncRNA). A distinct subgroup of lncRNAs is circular RNAs (circRNAs), which can be easily identified by unique junction reads, resulting from their biogenesis. CircRNAs are largely cytosolic and thought to either code for micro-peptides屈尊 发表于 2025-3-25 21:09:29
http://reply.papertrans.cn/23/2267/226632/226632_25.pngdiscord 发表于 2025-3-26 00:53:53
http://reply.papertrans.cn/23/2267/226632/226632_26.pngHERE 发表于 2025-3-26 05:43:47
http://reply.papertrans.cn/23/2267/226632/226632_27.pngAcquired 发表于 2025-3-26 09:39:09
State Regulation: Achieving Ascendancy,As (miRNAs) and inhibit the activity of miRNAs. Alternatively, miRNAs could also directly target circRNAs and regulate the expression of circRNAs. Here we describe the Argonaute2 (AGO2) RNA immunoprecipitation (RIP) and luciferase screening assays to identify the interaction between circRNAs and miR牲畜栏 发表于 2025-3-26 15:17:10
http://reply.papertrans.cn/23/2267/226632/226632_29.pngneologism 发表于 2025-3-26 19:46:29
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