流浪
发表于 2025-3-28 17:21:14
https://doi.org/10.1007/978-1-349-08714-3ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdow
Abnormal
发表于 2025-3-28 20:56:22
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Recessive
发表于 2025-3-29 01:03:21
Joaquín Bernáldez,Rocío Juliana Herrerag sequences to their cognate linear RNAs from the same gene loci, leading to difficulties in distinguishing them from each other. A recent study has shown that some circRNAs can be specifically depleted by using base editing systems to target their predominantly back-splice sites for circularization
十字架
发表于 2025-3-29 05:18:29
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使声音降低
发表于 2025-3-29 08:49:21
James D. Bradbury,Courtney Cox Smithrpart and can be designed for efficient expression in different cell and tissue types. In this chapter, we developed different backsplicing circRNA cassettes that can enable efficient gene expression in various cell and tissue types. Furthermore, we packaged cassettes encoding circRNAs into adeno-as
Mangle
发表于 2025-3-29 15:18:17
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Brocas-Area
发表于 2025-3-29 19:06:52
https://doi.org/10.1007/978-1-0716-3678-7splice-aware pseudo-alignment scheme; paired-end RNA-sequencing; ddPCR; CLIP-seq; Bioinformatics
Fluctuate
发表于 2025-3-29 20:42:24
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不朽中国
发表于 2025-3-30 00:39:35
Christoph Dieterich,Marie-Laure BaudetIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
迁移
发表于 2025-3-30 07:52:48
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