LAP 发表于 2025-3-27 00:19:38
Nanopore-Mediated Sequencing of Circular RNAircRNA are sequenced without prior circRNA enrichment. Both methods were originally described in Karim et al. (Rahimi et al., Nat Commun 12: 4825, 2021) where they were applied to characterize the exon-intron structure of >10.000 circRNAs in mouse and human brains.噱头 发表于 2025-3-27 02:48:16
http://reply.papertrans.cn/23/2267/226630/226630_32.pngBET 发表于 2025-3-27 06:11:26
Regulating Technological Innovation to higher levels than their cognate linear mRNAs, but the vast majority are expressed at low levels. Hence, for most circRNAs, only a handful of sequencing reads, if any, that span the backsplicing junction are observed in standard RNA-seq libraries. It thus has become common to use the 3′–5′ exonu事先无准备 发表于 2025-3-27 12:54:54
https://doi.org/10.1007/978-3-030-43527-1otic cells. Unlike canonical linear RNA molecules, circRNAs form a covalently closed continuous loop structure without a 5′ or 3′ end. They are generated by a process called back-splicing, in which a downstream splice donor site is joined to an upstream splice acceptor site. CircRNAs have been foundwreathe 发表于 2025-3-27 15:43:19
https://doi.org/10.1007/978-3-030-43527-1otential to modulate biological processes and are being investigated for their diagnostic and therapeutic applications. Circular RNAs (circRNAs), generated through back-splicing of exons, are enriched in EVs. Given their unique characteristics and diverse functions, EV-circRNAs are important playersDevastate 发表于 2025-3-27 19:48:01
http://reply.papertrans.cn/23/2267/226630/226630_36.png能量守恒 发表于 2025-3-27 22:00:10
Problems in Covert Investigationsies using high throughput RNA-sequencing (RNA-seq) techniques and novel computational programs reported the abundant and ubiquitous expression of circRNAs originating by pre-mRNA backsplicing. CircRNAs are mostly involved in gene expression by regulating functions of interacting microRNAs (miRNAs) aAGGER 发表于 2025-3-28 02:28:09
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http://reply.papertrans.cn/23/2267/226630/226630_39.pnglanguor 发表于 2025-3-28 12:25:44
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