充气女
发表于 2025-3-25 04:25:18
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CRUMB
发表于 2025-3-25 11:08:52
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Cardiac-Output
发表于 2025-3-25 11:41:24
Frank Schimmelfennig,Guido Schwellnusy tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.
压碎
发表于 2025-3-25 19:00:33
Regierungssysteme in Mittel- und Osteuropase, and therapies. However, the primary basal cells required to establish the ALI cultures generally lose their ability to differentiate by the second or third passage, requiring a fresh batch, which can be limiting, particularly from donors with rare genotypes or in studies where gene modification
阴谋小团体
发表于 2025-3-25 22:13:47
Grotz Florian,Ferdinand Müller-Rommelom their apical surface into the cerebrospinal fluid (CSF). This specialized layer of E1 cells constitutes the border between the CSF and the brain interstitial fluid (BIF), and by controlling influx and efflux across the CSF to BIF interface, it is increasingly recognized to play an integral role i
大吃大喝
发表于 2025-3-26 01:11:20
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aptitude
发表于 2025-3-26 07:19:22
Regierungssysteme in Mittel- und Osteuropa fluid. In cultures, this ciliary beating is not well coordinated or occurs in small focal areas so the resulting mucociliary transport (MCT) is only linear over short distances. We present a method which induces ciliated cells in cultures to align during growth. The cells align along the axis of a
ALLAY
发表于 2025-3-26 11:03:47
SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Inteetter understanding respiratory pathogen host-cell interactions in the airways, one approach is to instead grow and differentiate these cells at an air-liquid interface (ALI). This chapter provides the working protocols used in our lab for producing ALI cultures, infecting them with SARS-CoV-2 and monitoring viral replication.
使成整体
发表于 2025-3-26 14:04:13
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圣人
发表于 2025-3-26 20:18:07
Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging,living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.