陈旧 发表于 2025-3-27 00:59:52

Primary Human Nasal Epithelial Cell Culture,y tract. The current chapter details protocols from sample collection to culturing HNEC under air-liquid interface (ALI) conditions to generate well-differentiated, functional airway cultures including displaying beating motile cilia.

赔偿 发表于 2025-3-27 03:48:03

https://doi.org/10.1007/978-1-0716-3507-0Tissue development; Homeostasis; Ciliopathies; Ciliary proteins; Human disease; Toxicant exposure

Arrhythmia 发表于 2025-3-27 08:17:37

978-1-0716-3509-4The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

慢慢啃 发表于 2025-3-27 11:35:12

Vito MennellaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

Expressly 发表于 2025-3-27 14:47:39

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誓言 发表于 2025-3-27 19:22:49

Cilia978-1-0716-3507-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

Locale 发表于 2025-3-28 00:42:55

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即席 发表于 2025-3-28 05:51:24

Die Gauck-Behörde: das unmögliche Amttion and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.

IDEAS 发表于 2025-3-28 07:30:10

Wie gefestigt ist unsere Demokratie?living cells. Here, we describe experimental strategies for endogenous PCR-tagging of ciliary genes in human RPE1 cells and how image acquisition and analysis of the expressed fluorescently tagged proteins can be utilized to study the dynamic ciliary processes of intraflagellar transport and vesicular trafficking.

古老 发表于 2025-3-28 14:04:23

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