闷热
发表于 2025-3-23 11:17:06
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不遵守
发表于 2025-3-23 17:31:25
Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle to study the cell cycle-dependent dephosphorylation of pRb by various PP1 isozymes, the specificity of PP1 isozymes for distinct pRb phosphorylation sites, the dephosphorylation of pRb associated with apoptosis, and the cell cycle- and pRb-dependent phosphorylation of PP1.
内向者
发表于 2025-3-23 21:36:26
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CUMB
发表于 2025-3-23 22:10:50
Analyzing the Regulation and Function of ATMd with anti-ATM antibodies, and protein kinase activity is measured using p53.-specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed by the procedures outlined above.
纬度
发表于 2025-3-24 03:14:44
Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells. We report here a protocol for purification of an inhibitor of the APC/C from HeLa cells, called mitotic checkpoint complex (MCC). Our procedure is based on biochemical purification and characterization of the APC/C inhibitory activity from extracts of HeLa cells.
instate
发表于 2025-3-24 10:27:55
Analysis of the Spindle-Assembly Checkpoint in HeLa Cellses and spindle tension, chromosome positioning, and kinetochore signaling by the Mad2 or Bub1 checkpoint proteins. We also describe a bi-parameter flow cytometric assay, using either MPM-2 or anti-phospho-(Ser10)-histone H3 antibodies, for quantitating mitotic cells.
DOLT
发表于 2025-3-24 11:52:29
Introduction to Flex Applications, to study the cell cycle-dependent dephosphorylation of pRb by various PP1 isozymes, the specificity of PP1 isozymes for distinct pRb phosphorylation sites, the dephosphorylation of pRb associated with apoptosis, and the cell cycle- and pRb-dependent phosphorylation of PP1.
神刊
发表于 2025-3-24 17:27:53
Introduction to Flex Applications,in immune-complex kinase assays. This assay involves isolation of the kinase by immunoprecipitation and the in vitro phosphorylation of a specific substrate in the presence of radio-labeled ATP. Here we describe, in detail, the determination of PIKK catalytic activity with a standardized immune-complex kinase assay protocol.
Friction
发表于 2025-3-24 22:22:54
https://doi.org/10.1007/978-1-4302-1836-4d with anti-ATM antibodies, and protein kinase activity is measured using p53.-specific substrate or by immunoblotting extracts with an anti-phosphoserine 15 p53-specific antibody. Missense mutations affecting ATM kinase activity are detected using in vitro mutagenesis of ATM cDNA followed by the procedures outlined above.
保留
发表于 2025-3-25 00:40:37
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