喊叫
发表于 2025-3-30 11:08:55
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debase
发表于 2025-3-30 13:11:16
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CULP
发表于 2025-3-30 18:09:14
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Ascendancy
发表于 2025-3-30 22:11:35
Assaying Cdc25 Phosphatase Activity25 proteins produced in . or insect cells, a fluorimetric assay using fluorescein diphosphate (FDP) as a substrate is recommended. To analyze endogenous Cdc25 phosphatase activities of immuno-precipitates from total cellular extracts, the physiological substrate Cdk1/cyclin B1 is most sensitive.
Petechiae
发表于 2025-3-31 04:08:38
Analyzing the Regulation and Function of ATMned into an inducible EBV-based vector (pMEP4) and its expression analyzed in a stably transfected cell line. ATM protein induction is monitored by immunoblotting with antibodies against both ATM and a FLAG sequence tag in the recombinant protein. Extracts from irradiated cells are immunoprecipitate
venous-leak
发表于 2025-3-31 07:32:30
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节约
发表于 2025-3-31 12:52:43
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Accolade
发表于 2025-3-31 16:54:55
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晚间
发表于 2025-3-31 19:18:57
Analysis of the Spindle-Assembly Checkpoint in HeLa Cellsachment or spindle tension. Here, we detail procedures for the analysis of the spindle-assembly checkpoint in adherent mammalian cells. These techniques focus on pharmacological approaches and immunofluorescence microscopy to verify the state of spindle assembly, kinetochore attachment of microtubul
用不完
发表于 2025-4-1 00:43:08
Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assayy attached to the mitotic spindle, this checkpoint blocks the activity of a large ubiquitin protein ligase complex, called the anaphase-promoting complex (APC) or cyclosome. This chapter describes the detailed protocols of an in vitro ubiquitination assay reconstituted with purified APC, cofactors,