有角
发表于 2025-4-1 02:11:34
Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalics, endocytosis, exocytosis, and cytoskeletal reorganization. It is desirable to visualize these events without interference from other regions deeper within the cell. Total internal reflection fluorescence (TIRF) microscopy utilizes an elegant optical sectioning approach to visualize fluorophores n
Tailor
发表于 2025-4-1 09:06:29
Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryoni what complexes are present in a cell, but information about the diversity of individual protein complexes is lost. Here, we describe single-cell, single-molecule pull-down (sc-SiMPull), a TIRF microscopy-based coimmunoprecipitation method, to visualize thousands of individual proteins, their bindin
Antagonist
发表于 2025-4-1 13:45:36
Biochemical Assays to Detect Activation of Small GTPases Rho, Rac , and Cdc42 during Morphogenesis,the small GTPases Rho, Rac, and Cdc42, is one key mechanism that regulates cell polarity and migration during vertebrate gastrulation. In this chapter, we describe biochemical assays for detection of Wnt/Fz-mediated activation of Rho, Rac and Cdc42 in both mammalian cells and . embryo explants.
合并
发表于 2025-4-1 17:00:39
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名字
发表于 2025-4-1 18:34:20
Regulation of Cell Polarity by Posttranslational Protein Palmitoylation, diseases. Therefore, the signaling pathways involved in establishing and maintaining cell polarity are tightly controlled. Protein S-palmitoylation has been recently recognized as an important posttranslational modification involved in cell polarity, via dynamic covalent attachment of fatty acyl gr
COWER
发表于 2025-4-2 01:43:23
Enrichment and Detection of Wnt Proteins from Cell Culture Media, signaling range are under tight cellular control such that detection of Wnt proteins in biological samples is often extremely difficult. In this chapter, we describe a protocol to detect secreted Wnt proteins in the culture medium of cell lines that ectopically or endogenously express Wnt genes. Th
半球
发表于 2025-4-2 05:12:38
Using Live Imaging to Examine Early Cardiac Development in Zebrafish,y. Similarly, live imaging can be vital to elucidating the pathology of genetic disorders and diseases. Model systems such as zebrafish, whose in vivo development is accessible to both the microscope and genetic manipulation, are particularly well-suited to the use of live imaging. Here we describe