友好
发表于 2025-3-23 10:23:50
La gestione dei momenti critici,on levels and patterns. Bacterial based recombination system can be used to efficiently introduce mutations, fluorescent protein tags, and LoxP sites for conditional expressions. Cre can also be inserted into BACs to map the contribution of cells expressing any PCP gene of interest, and study PCP mediated tissue morphogenesis.
滑动
发表于 2025-3-23 17:53:43
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不朽中国
发表于 2025-3-23 18:02:16
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Harness
发表于 2025-3-23 22:22:06
BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mion levels and patterns. Bacterial based recombination system can be used to efficiently introduce mutations, fluorescent protein tags, and LoxP sites for conditional expressions. Cre can also be inserted into BACs to map the contribution of cells expressing any PCP gene of interest, and study PCP mediated tissue morphogenesis.
Carbon-Monoxide
发表于 2025-3-24 05:01:55
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PHIL
发表于 2025-3-24 10:32:51
1064-3745expertsThis volume explores detailed methods to investigate various aspects of biology related to cell polarity, or asymmetry within a cell. Molecular, cellular, and tissue-level regulation and function as well as diseases caused by impairment of cell polarity are explored by these methods. Beginni
浮夸
发表于 2025-3-24 10:53:18
Matrixmethoden der paraxialen Optik,it relatively slow junctional turnover. We provide a step-by-step practical guide to performing FRAP, and list a series of controls and optimizations to do before conducting a FRAP experiment. Finally, we describe how to present the FRAP data for publication.
Cloudburst
发表于 2025-3-24 17:02:15
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Rct393
发表于 2025-3-24 22:37:53
Erzeugung von polarisiertem Licht,gle-molecule pull-down (sc-SiMPull), a TIRF microscopy-based coimmunoprecipitation method, to visualize thousands of individual proteins, their binding partners, and protein complex stoichiometry directly from single-cell lysate. By iterating sc-SiMPull over time, temporal dynamics of protein complexes in response to signaling can be constructed.
eardrum
发表于 2025-3-25 01:17:39
Interferenz an Mehrfachschichten,as been recently recognized as an important posttranslational modification involved in cell polarity, via dynamic covalent attachment of fatty acyl groups to the cysteine residues of cell polarity proteins. Here, we describe the methods to study the function and regulation of S-palmitoylation of cell polarity proteins.