渗入 发表于 2025-4-1 04:51:58

The Lowry Method for Protein Quantitation,itive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (.) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely ac

foodstuff 发表于 2025-4-1 06:00:30

The Bicinchoninic Acid (BCA) Assay for Protein Quantitation,to Cu. under alkaline conditions (..). The Cu. is then detected by reaction with BCA. The two assays are of similar sensitivity, but since BCA is stable under alkali conditions, this assay has the advantage that it can be carried out as a one-step process compared to the two steps needed in the Lowr

弯曲的人 发表于 2025-4-1 13:03:47

The Bradford Method for Protein Quantitation, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of b

Provenance 发表于 2025-4-1 14:20:02

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向下 发表于 2025-4-1 21:15:05

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查看完整版本: Titlebook: Basic Protein and Peptide Protocols; John M. Walker Book 1994 Humana Press 1994