鸟笼
发表于 2025-3-23 13:33:32
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小丑
发表于 2025-3-23 14:13:35
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Antioxidant
发表于 2025-3-23 21:15:47
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dime the very high electroendosmotic flow caused by charged groups on the glass walls of the gel tubes. As a consequence, pH gradients are unstable and rarely extend above pH 8.0, leading to loss of basic proteins from 2-D maps. Gradients can be extended to pH 10 by special treatment of the glass IEF tubes (.) or by using horizontal flat-bed IEF (..).
BLAND
发表于 2025-3-24 01:44:15
Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the Firstsing (IEF) gels containing 9. urea and 2% (w/v) of the detergent NP-40 with discontinuous polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE) in the second dimension. This technique is described in . and is reviewed in refs. .–..
Maximize
发表于 2025-3-24 05:20:27
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prosthesis
发表于 2025-3-24 07:56:30
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Urea508
发表于 2025-3-24 13:59:40
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肌肉
发表于 2025-3-24 18:54:40
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MITE
发表于 2025-3-24 19:07:46
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裂口
发表于 2025-3-25 00:28:28
Financial Aspects of HCs Business Models,taining of proteins following gel electrophoresis was a major advance providing a detection sensitivity between 20–200 times higher than methods using CBB R-250, being able to detect about 0.1 ng protein/band.