Coolidge 发表于 2025-3-21 19:39:39
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Liposomal Permeabilization Assay to Study the Functional Interactions of the BCL-2 Family, be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function of确保 发表于 2025-3-22 01:16:47
Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs, a dominant inhibitor of programmed cell death and a major anticancer drug target. The protocols are relatively straightforward. Provided care is taken to ensure protein integrity and sample homogeneity, BCL-XL can be readily reconstituted in nanodiscs, with its hydrophobic C-terminal tail anchoredjudiciousness 发表于 2025-3-22 06:42:54
1064-3745 essary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .BCL-2 978-1-4939-8861-7Series ISSN 1064-3745 Series E-ISSN 1940-6029胆小懦夫 发表于 2025-3-22 10:59:12
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Heather Winskel,Theeraporn Ratitamkul be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function ofAmenable 发表于 2025-3-22 19:19:20
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Liquid Metal Biomedical Imaging,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.rheumatism 发表于 2025-3-23 06:03:51
Application of Mito-Priming to Generate BCL-2 Addicted Cells,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.