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Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactfectious virus can be produced if purified poxvirus DNA is transfected into cells previously infected with another poxvirus. This process is termed . if the infecting virus is different from the transfected virus. We describe a method in which the high-frequency recombination and replication reactio男生戴手铐 发表于 2025-3-29 08:03:05
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Growing Poxviruses and Determining Virus Titer,gs. The main principle of isolating the virus is to mechanically lyse infected cells. The virus can then be semipurified by centrifugation through a sucrose cushion. Further purification can be achieved with a sucrose gradient. This chapter describes methods for the preparation of large-scale growthRodent 发表于 2025-3-29 20:00:53
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Orthopoxvirus Diagnostics, amplification, with either restriction cleavage or sequencing of amplicons, has been gaining credibility as a more rapid, specific, sensitive, and often cost-saving technique for research and diagnostic laboratories. This chapter is consolidated using prior research papers from our laboratories witChagrin 发表于 2025-3-30 05:05:42
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