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Construction of cDNA Libraries in Vaccinia Virus,st commonly used method to construct recombinant poxvirus is homologous recombination. The frequency of recombinants derived in this manner is of the order of 0.1%, sufficient to recover a recombinant of a purified DNA clone in a transfer plasmid, but far too low to permit construction of a represenCupping 发表于 2025-3-22 02:38:28
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Monitoring of Human Immunological Responses to Vaccinia Virus,imple enzyme-linked immunosorbent assay (ELISA) to quantify vaccinia virus antibody titer. Additionally, to define serum-neutralizing activity, both a classical plaque reduction assay and a high-throughput 96-well plate method based on reduction of recombinant vaccinia virus expressed β-galactosidas禁止 发表于 2025-3-22 10:55:03
Vaccinia Virus as a Tool for Immunologic Studies,C class II-restricted antigen processing and presentation owing to many reasons specified in this chapter. This chapter aims to describe the commonly used procedures in this field that employ vaccinia virus systems, particularly troubleshooting common problems encountered during experiments.词汇 发表于 2025-3-22 16:45:01
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