强制令
发表于 2025-3-26 22:20:48
Selection of Aptamers Against Whole Living Cells: From Cell-SELEX to Identification of Biomarkers assays. Furthermore, we also provide a protocol to purify and identify the target of these aptamers. These protocols could be useful for the discovery of lead therapeutic compounds and diagnostic cell-surface biomarkers.
描述
发表于 2025-3-27 01:25:43
Book 2017g, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and
Permanent
发表于 2025-3-27 07:47:05
Generating Conformation and Complex-Specific Synthetic Antibodiesntibodies for future therapeutic use. The methods described are also applicable to screening directly on cells and can be ported to other in vitro directed evolution systems utilizing non-immunoglobulin scaffolds.
Kidney-Failure
发表于 2025-3-27 13:06:41
http://reply.papertrans.cn/89/8844/884327/884327_34.png
NAV
发表于 2025-3-27 17:38:12
http://reply.papertrans.cn/89/8844/884327/884327_35.png
检查
发表于 2025-3-27 18:11:12
http://reply.papertrans.cn/89/8844/884327/884327_36.png
LATHE
发表于 2025-3-28 01:25:11
http://reply.papertrans.cn/89/8844/884327/884327_37.png
Cardioversion
发表于 2025-3-28 03:27:24
Whole Cell Panning with Phage Displaye panning), whole cell panning is an elegant way to present membrane embedded target antigens in their natural environment and conformation to antibody-bearing phages. Here, a whole cell panning procedure using a Fab-based antibody library including primary cell based screening for selectivity is described.
Obituary
发表于 2025-3-28 07:53:04
http://reply.papertrans.cn/89/8844/884327/884327_39.png
公式
发表于 2025-3-28 10:50:08
A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteinsstuck in the endosomes and those that have escaped into the cytosol. Here, we describe a method employing the prokaryotic enzyme biotin ligase that overcomes this problem and which can be employed for a highly sensitive quantification of cytosolic protein delivery.