GLEAN 发表于 2025-3-26 23:48:53
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Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermoere expressed in . and screened for enhanced thermostability by a filter assay and by temperature-gradient gel electrophoresis (TGGE). Characterization of selected mutants and application of TGGE to investigate the thermal stability of proteases and protease-inhibitor complexes in general is described.BARGE 发表于 2025-3-27 06:32:39
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Advances in Experimental Medicine and Biologyhttp://image.papertrans.cn/t/image/881444.jpg腐败 发表于 2025-3-27 22:03:33
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Subtilases: Subtilisin-Like Serine ProteasesSubtilases are members of the family of subtilisin-like serine proteases. Over 70 subtilases are presently known, more than 60 of which with their complete amino acid sequence. In this update, details of more than 20 new subtilases discovered recently are summarized, and their sequences included in a multiple sequence alignment table.d-limonene 发表于 2025-3-28 09:20:20
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Propeptide-Mediated Folding in Subtilisin: The Intramolecular Chaperone Concepte enzyme was named ‘subtilisin’. Different variants of this enzyme have been identified and well studied. Among these include subtilisin BPN’, subtilisin E and subtilisin Carlsberg. Later this exoenzyme was found to belong to a very large family of proteins spanning both prokaryotes and eukaryotes called subtilases..