枯萎将要 发表于 2025-3-25 04:14:37

Crystal Structure Analysis of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stabilitylity have been determined. The simplest variant, S3, contains two altered residues, N218S and S221C. The N218S change was incorporated for its stabilizing effects and its influence on crystallization; the S221C change, a modification of the active site serine, was included to reduce autolysis. The s

GLUT 发表于 2025-3-25 08:12:28

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阻碍 发表于 2025-3-25 12:43:37

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Thrombolysis 发表于 2025-3-25 17:27:20

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NIB 发表于 2025-3-25 20:07:18

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凝乳 发表于 2025-3-26 02:49:33

Studies of Binding Sites in the Subtilisin from , by Means of Site Directed Mutagenesis and Kinetic s using systematic variations of substrate structures. The recent development of highly efficient donor/acceptor pairs for substrates based on intramolecular fluorescence quenching, allowing the use of long peptide substrates spanning the entire binding site, represents a significant improvement in this context.

钳子 发表于 2025-3-26 08:11:36

Crystal Structure Analysis of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stabilityl site specific mutations, T22C and S87C, which form a stabilizing disulfide bridge. The structural changes and influence on stability of each of these mutations are discussed in the context of supporting biophysical studies.

Mhc-Molecule 发表于 2025-3-26 09:01:02

0065-2598 n is the commercial utility of this class of proteases. The subtilisin symposium was the first international meeting to bring together a large number of groups that have focused on the subtilisins and the subtilases-the protein superfamily of subtilisin-like enzymes. The results presented at the sym

Conserve 发表于 2025-3-26 14:42:02

The Complex Between Mesentericopeptidase and Eglin-Cture to our needs. The present paper describes the structure of mesentericopeptidase (henceforth SBMEP) in its 1:1 complex with the leech inhibitor eglin-C at 2.0 Å resolution. The structure of the complex has been previously published in full. and we here briefly summarize the major points of the analysis.

Decrepit 发表于 2025-3-26 20:46:17

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查看完整版本: Titlebook: Subtilisin Enzymes; Practical Protein En Richard Bott,Christian Betzel Book 1996 The Editor(s) (if applicable) and The Author(s), under exc