不断的变动
发表于 2025-3-25 04:07:23
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终止
发表于 2025-3-25 10:16:18
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DEI
发表于 2025-3-25 14:44:07
Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins,ilibria. Here we describe the membrane-enabled hybrid-solvent continuous constant pH molecular dynamics method for capturing atomic details of proton-coupled conformational dynamics of transmembrane proteins. Example protocols from our recent application studies of proton channels and ion/substrate transporters are discussed.
photophobia
发表于 2025-3-25 17:29:57
NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplifd for the recombinant production of stable-isotope labeled hVDAC1 in ., the isolation of hVDAC1 from inclusion bodies and the refolding into detergent micelles, as well as the reconstitution of hVDAC1 into lipids to form 2D crystals.
vascular
发表于 2025-3-25 23:11:24
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affinity
发表于 2025-3-26 00:29:19
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记忆
发表于 2025-3-26 07:03:42
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密切关系
发表于 2025-3-26 11:51:43
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chastise
发表于 2025-3-26 13:21:49
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overrule
发表于 2025-3-26 17:34:41
,Biochemical Characterization of GPCR–G Protein Complex Formation,ence of agonist or antagonist, as well as the complex dissociation in the presence of GTP analogue. Methods used in the protocol are affinity purification and gel filtration, which are also commonly used in protein sample preparation for structural work. Therefore, the protocol can be easily adapted for large-scale sample preparation.