小鹿
发表于 2025-3-30 11:56:54
Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy,ystal contacts. In the cases where the protein lacks surface patches conducive to such interactions, crystallization may not occur. However, it is possible to enhance the likelihood of crystallization by engineering such patches through site-directed mutagenesis, targeting specifically residues with
Shuttle
发表于 2025-3-30 15:27:42
Data Collection for Crystallographic Structure Determination, optimized for a particular application, make the structure solution and refinement easier and enhance the accuracy of the final models. This chapter describes the principles of the rotation method of data collection and discusses various scenarios that are useful for different types of applications
生气的边缘
发表于 2025-3-30 17:12:27
Structure Determination, Refinement, and Validation,ds, software and hardware, crystallographic structure determination no longer requires a specialist in the method, but rather it has become a technique that can be readily applied to many research problems. The high-throughput approaches developed and used by structural genomics projects can be adap
Afflict
发表于 2025-3-31 00:28:07
Virtual High-Throughput Ligand Screening, protein structures. Using a variety of publicly available software tools, it is possible to computationally model, predict, and evaluate how different ligands interact with a given protein. At the Center for Structural Genomics of Infectious Diseases (CSGID) a series of protein analysis, docking an
去才蔑视
发表于 2025-3-31 04:44:37
Book 2014 to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the highly successful .Methods in Molecular Biology
Patrimony
发表于 2025-3-31 05:03:05
Structural Genomics of Human Proteins,ecade or more of work in this field has identified optimal experimental strategies that can be used to expedite expression and crystallization of human proteins—and we provide some guidance to this end.
seroma
发表于 2025-3-31 09:10:40
Target Selection for Structural Genomics of Infectious Diseases, experimental strategies that explore the effects of knocking out the protein. Publicly available computational tools have been cited as much as possible. Where these do not exist, the concepts underlying in-house tools developed for the Center for Structural Genomics of Infectious Diseases have been described.
BLA
发表于 2025-3-31 16:34:30
Oxidative Refolding from Inclusion Bodies,efolding buffer containing arginine and a mixture of oxidized and reduced glutathione, recovery of the recombinant protein using a stirred cell concentrator, and removal of the aggregated or misfolded fraction by passage over size-exclusion chromatography. The quality of the resulting protein can be assessed by SDS-PAGE.
Cupidity
发表于 2025-3-31 18:33:35
http://reply.papertrans.cn/88/8800/879922/879922_59.png
Noisome
发表于 2025-4-1 01:38:11
http://reply.papertrans.cn/88/8800/879922/879922_60.png