爱得痛了 发表于 2025-3-28 15:01:33
Expression and Solubility Testing in a High-Throughput Environment, For many applications, . remains the most widely used expression system due to the relative ease of adapting it to HT pipelines. Herein is described a platform using a 96-well format for efficient expression and solubility screening of target proteins.纺织品 发表于 2025-3-28 22:21:28
Protein Production for Structural Genomics Using , Expression,crystallography and NMR are the two best methods for atomic level structure determination. However, these methods require milligram quantities of proteins. In this chapter a reproducible methodology for large-scale protein production applicable to a diverse set of proteins is described. The approachJuvenile 发表于 2025-3-29 01:30:26
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Salvage or Recovery of Failed Targets by In Situ Proteolysis,cromolecular complexes. The simplicity of the procedure and high recovery rate make it a method of first choice for recalcitrant targets. An improved and updated in situ proteolysis protocol used in high-throughput structural biology platforms is described.反话 发表于 2025-3-30 04:39:45
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