ATP861
发表于 2025-3-25 04:39:27
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mechanism
发表于 2025-3-25 08:48:15
Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropyion extended to 2.1 Å resolution along a* and c* directions but only to 3.0 Å along the b* direction. Severe anisotropy such as this is often associated with lack of detail in electron density maps, stalled model improvement, and poor refinement statistics. Published methods for overcoming these dif
无能的人
发表于 2025-3-25 13:58:16
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落叶剂
发表于 2025-3-25 19:54:38
A Cost-Effective Protocol for the Parallel Production of Libraries of 13CH3-Specifically Labeled Mutcombined with improved NMR spectroscopy enable nowadays studies of proteins complexes up to 1 MDa. For such large complexes, the major interest lies in obtaining structural, dynamic and interaction information in solution, which requires sequence-specific resonance assignment of NMR signals. While s
凶猛
发表于 2025-3-25 23:59:38
High-Throughput Expression Screening and Purification of Recombinant Proteins in ,cale, there is the potential to use the purified proteins for characterization in pilot studies, for sensitive micro-assays, or for the quick detection of and differentiation of the expected size and oxidation state of the protein by mass spectrometry.
BIAS
发表于 2025-3-26 01:34:01
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开始没有
发表于 2025-3-26 07:55:17
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声明
发表于 2025-3-26 11:05:46
Methods to Refine Macromolecular Structures in Cases of Severe Diffraction Anisotropyof anisotropy, and then applies ellipsoidal resolution boundaries, anisotropic scaling, and B-factor sharpening to the data set to compensate for the deleterious effects of diffraction anisotropy. Here, I offer advice on implementing these methods to facilitate refinement of macromolecular structures in cases of severely anisotropic data.
似少年
发表于 2025-3-26 13:05:06
Expression Screening in Mammalian Suspension Cellstions are varied to identify the optimal expression conditions. This requires multi-parallel expression screening in mammalian cells similar to that already described for .. . expression. Here we describe in detail a multi-parallel method to express proteins in mammalian suspension cells by transient transfection in 24-well blocks.
ostensible
发表于 2025-3-26 18:12:23
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