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发表于 2025-3-26 22:31:59
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orthodox
发表于 2025-3-27 02:15:48
https://doi.org/10.1007/978-1-0716-0892-0Protein production; Structural analyses; Data management; Protein modeling; Computational tools; Structur
Enzyme
发表于 2025-3-27 09:11:56
978-1-0716-0894-4Springer Science+Business Media, LLC, part of Springer Nature 2021
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发表于 2025-3-27 11:01:34
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生气地
发表于 2025-3-27 14:41:53
Yu Wai Chen,Chin-Pang Bennu YiuIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
outskirts
发表于 2025-3-27 20:58:43
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kyphoplasty
发表于 2025-3-28 01:15:42
Rational Design and Construction of Active-Site Labeled Enzymesimportant task in structural genomics. To characterize the function of an enzyme at molecular level, placing a reporter on the active site of an enzyme can be a strategy to examine the dynamics of the interaction between enzyme and its substrate/inhibitor. In this chapter, we describe an approach of
无情
发表于 2025-3-28 02:56:05
Screening and Production of Recombinant Human Proteins: Ligation-Independent Cloningl-expressed, soluble, and stable protein and one that crystallizes and diffracts to a sufficient resolution for structural determination. At the Structural Genomics Consortium, we opted for the ligation-independent cloning (LIC) method which provides the throughput we desire to produce and screen ma
Culpable
发表于 2025-3-28 07:14:54
Screening and Production of Recombinant Human Proteins: Protein Production in sing LIC. In this chapter we provide a step-by-step procedure of our . system for test expressing intracellular (soluble) proteins in a 96-well format that enables us to identify which proteins or truncated versions are expressed in a soluble and stable form suitable for structural studies. In addit
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发表于 2025-3-28 13:51:42
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