correspondent 发表于 2025-3-26 22:05:41
The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the high-salt recovered sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are mad凝视 发表于 2025-3-27 03:10:12
http://reply.papertrans.cn/88/8735/873498/873498_32.pngear-canal 发表于 2025-3-27 08:54:39
Simultaneous Quantification of Spatial Genome Positioning and Transcriptomics in Single Cells with scification. Yet it is still an outstanding question in the field what the direct impact of spatial genome organization on gene expression is. To elucidate this relationship further, we have recently developed scDam&T-seq, a method that simultaneously quantifies protein–DNA interactions and transcrip是限制 发表于 2025-3-27 12:44:54
High-Throughput DNA FISH (hiFISH)nd analysis to visualize and localize tens to hundreds of genomic loci in up to millions of cells. hiFISH can be used to measure physical distances between pairs of genomic loci, radial distances from genomic loci to the nuclear edge or center, and distances between genomic loci and nuclear structurpalliate 发表于 2025-3-27 13:43:27
http://reply.papertrans.cn/88/8735/873498/873498_35.png慷慨不好 发表于 2025-3-27 19:27:10
http://reply.papertrans.cn/88/8735/873498/873498_36.png提名的名单 发表于 2025-3-27 21:58:23
http://reply.papertrans.cn/88/8735/873498/873498_37.pngprojectile 发表于 2025-3-28 04:59:29
http://reply.papertrans.cn/88/8735/873498/873498_38.pngpus840 发表于 2025-3-28 06:43:18
High-Throughput DNA FISH (hiFISH)FISH to measure distances between genomic loci, including all steps involved in DNA FISH probe design and preparation, cell culture, DNA FISH staining in 384-well imaging plates, automated image acquisition and analysis, and, finally, statistical analysis.Landlocked 发表于 2025-3-28 14:22:15
Assessment of Multiway Interactions with Tri-C investigate how multiple .-regulatory elements interact together in higher-order structures in single nuclei and address questions regarding structural cooperation between these elements. Here, we describe the procedure for designing and performing a Tri-C experiment.