GAVEL
发表于 2025-3-23 10:37:26
http://reply.papertrans.cn/87/8686/868501/868501_11.png
nepotism
发表于 2025-3-23 14:56:36
http://reply.papertrans.cn/87/8686/868501/868501_12.png
entice
发表于 2025-3-23 18:27:55
Direct Cloning of Double-Stranded RNAsMost annotated genomes show a large number of sense–antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.
Acetaldehyde
发表于 2025-3-24 00:17:17
http://reply.papertrans.cn/87/8686/868501/868501_14.png
表被动
发表于 2025-3-24 03:54:05
978-1-4939-4903-8Springer Science+Business Media New York 2015
抱怨
发表于 2025-3-24 10:21:01
Small Non-Coding RNAs978-1-4939-2547-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
CRATE
发表于 2025-3-24 13:58:56
http://reply.papertrans.cn/87/8686/868501/868501_17.png
Multiple
发表于 2025-3-24 15:20:54
Alcoholic Precipitation of Small Non-Coding RNAsa standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.
Abrupt
发表于 2025-3-24 20:55:26
http://reply.papertrans.cn/87/8686/868501/868501_19.png
丑恶
发表于 2025-3-25 00:39:12
Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAsod is divided in two steps. First, RNA is reverse-transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR. This fast and simple method provides quantitative data with high sensitivity and specificity to study miRNAs and their functions.