JOG
发表于 2025-3-26 21:22:42
Flow Cytometric and Immunohistochemical Follow-Up of Spermatogonial Lineage Commitment,croenvironment and predicting progress of spermatogenesis. Here we define the evaluation of mouse male germ cell microenvironment at single cell and whole tissue section levels by using flow cytometric and immunohistochemical approaches.
IRS
发表于 2025-3-27 02:39:32
1064-3745experts.This updated edition explores techniques in identifying and characterizing various skin stem cells and generating skin-like structures in vitro. The goal of the book is to present exciting advances in skin biology in an effort to provide a better understanding of skin and, eventually, to al
Ccu106
发表于 2025-3-27 06:32:17
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TOM
发表于 2025-3-27 11:17:53
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教育学
发表于 2025-3-27 15:34:40
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Mangle
发表于 2025-3-27 18:42:29
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溃烂
发表于 2025-3-28 00:11:28
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供过于求
发表于 2025-3-28 02:51:45
Isolation, Characterization, and Utilization of Human Skin Basal and Suprabasal Epidermal Stem Cellcompositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.
Harass
发表于 2025-3-28 07:24:51
Isolation of Human Progenitor Epidermal Cells on Collagen Type IV and Analysis of Their Markers wit incubation with dispase followed by cell isolation for epidermis by digestion with trypsin. Cell isolated with this method can be seeded on collagen IV-coated dishes. The methods of analysis of epidermal stem cells markers (e.g., CD71, CD29) with flow cytometry and RT-PCR are also included.
讨好女人
发表于 2025-3-28 10:24:43
Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluoreschair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells located in the bulge. Three-dimensional and high-resolution imaging of these structures using whole-mount immunofluorescent staining allows to better characterize epidermal progenitors and stem cells.