abolish
发表于 2025-3-26 22:50:24
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史前
发表于 2025-3-27 01:48:53
Fluorescent Single-Stranded DNA-Binding Proteins Enable In Vitro and In Vivo Studies,hemical mechanism and biological function. Here, we describe the purification of fluorescent SSB chimeras with a unique number of fluorescent subunits incorporated per tetramer. We describe the use of these tetramers to enable clear visualization of SSB in vivo. Purified chimeras also facilitate sin
Ergots
发表于 2025-3-27 09:10:09
Use of Fluorescently Tagged SSB Proteins in In Vivo Localization Experiments,s information is through fluorescence microscopy. The abundance of .ingle-.trand .inding protein, SSB, at the replication fork makes it a good reporter of DNA replication. In this chapter I describe how to observe replication of the . chromosome in a strain that synthesizes a fluorescent derivative
interior
发表于 2025-3-27 10:23:28
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俗艳
发表于 2025-3-27 16:10:24
,Methods for Analysis of SSB–Protein Interactions by SPR,ts. Essentially, the method entails immobilizing a biotinylated single-stranded DNA oligo onto the chip surface, which is then bound by SSB prior to analyte addition to the SSB-coated chip. This allows for rapid qualitative and detailed quantitative analysis of both equilibrium and kinetic parameters of the SSB–protein interaction.
媒介
发表于 2025-3-27 20:17:43
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armistice
发表于 2025-3-28 00:02:46
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generic
发表于 2025-3-28 06:05:09
,Investigation of Protein–Protein Interactions of Single-Stranded DNA-Binding Proteins by Analyticalnguished from free binding partners by its different sedimentation behavior. As an example, we show how sedimentation velocity experiments of purified proteins can be employed to determine the binding parameters of the interaction of SSB and the χ subunit of DNA polymerase III from ..
elucidate
发表于 2025-3-28 06:28:06
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TRUST
发表于 2025-3-28 10:53:56
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