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Enzymatic Dispersion of Heart and Other Tissuesfor the patch clamp technique. They have a cell surface stripped of connective tissue, a prerequisite for the formation of a gigaseal between the current-recording pipette and the cell membrane (Neher, 1982).fatuity 发表于 2025-3-22 03:50:29
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Geometric Parameters of Pipettes and Membrane Patchesof the optical microscope. Only rarely is it possible to “see” the membrane patch during the experiment, and even then quantitative evaluation of its size is impossible or difficult. The purpose of this chapter is to provide some hints toward estimation of the tip geometry of patch pipettes and of pwhite-matter 发表于 2025-3-22 14:43:31
Science and Technology of Patch-Recording Electrodes tightly to the tip of a glass microelectrode. Because the seal is electrically tight, electrical noise is reduced by several orders of magnitude. Because the glass-membrane bond is mechanically strong, the patch of membrane spanning the tip may be isolated from the cell, or the patch may be ruptureSilent-Ischemia 发表于 2025-3-22 19:15:40
Enzymatic Dispersion of Heart and Other Tissueserry and Friend, 1969; cardiac myocytes: Vahouny ., 1970; smooth muscle cells: Bagby ., 1971). Most of the procedures have been designed to meet the needs of biochemical investigations. For electrophysiologists, enzymatic dissociation is of interest if the cells are not easily accessible otherwise o泛滥 发表于 2025-3-22 23:54:59
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