Indelible 发表于 2025-3-23 10:13:55
Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microscof kinetics and dynamics of single molecules in living cells. However, sample preparation is equally important for the successful single-molecule imaging. Here, we describe the equipment of TIRFM as well as the procedures for sample preparations for the single-molecule imaging in living cultured cells.Extricate 发表于 2025-3-23 17:25:10
Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking, molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.PAN 发表于 2025-3-23 18:02:52
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Book 2020patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by qobstruct 发表于 2025-3-24 07:21:15
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Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependee combined with optogenetics enables us to reveal neuronal activity-dependent dynamics of transcription factors in living cortical neurons. Here, we describe the detailed experimental procedures to study the transcriptional activity with physiological stimulation in living CNS neurons.ASSAY 发表于 2025-3-24 15:11:22
Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microsconsport a variety of cellular components. Translocation of motor proteins would be highly regulated, since various cargos are transported to their appropriate destinations. Single-molecule investigation of dynamic movement of motor proteins in living cells has enabled us to explore these regulatory mpuzzle 发表于 2025-3-24 21:15:47
In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins and ex vivo systems via biochemical and electrophysiological methods. With the emergence of single-molecule (SM) fluorescence microscopy techniques, it is now possible to characterize the molecular organization and the biophysical dynamics of ion channels in cells with precisions on the order of a f道学气 发表于 2025-3-25 03:03:56
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