Indelible 发表于 2025-3-23 10:13:55

Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microscof kinetics and dynamics of single molecules in living cells. However, sample preparation is equally important for the successful single-molecule imaging. Here, we describe the equipment of TIRFM as well as the procedures for sample preparations for the single-molecule imaging in living cultured cells.

Extricate 发表于 2025-3-23 17:25:10

Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking, molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.

PAN 发表于 2025-3-23 18:02:52

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捐助 发表于 2025-3-23 23:58:57

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refraction 发表于 2025-3-24 05:29:31

Book 2020patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by q

obstruct 发表于 2025-3-24 07:21:15

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APNEA 发表于 2025-3-24 13:07:28

Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependee combined with optogenetics enables us to reveal neuronal activity-dependent dynamics of transcription factors in living cortical neurons. Here, we describe the detailed experimental procedures to study the transcriptional activity with physiological stimulation in living CNS neurons.

ASSAY 发表于 2025-3-24 15:11:22

Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microsconsport a variety of cellular components. Translocation of motor proteins would be highly regulated, since various cargos are transported to their appropriate destinations. Single-molecule investigation of dynamic movement of motor proteins in living cells has enabled us to explore these regulatory m

puzzle 发表于 2025-3-24 21:15:47

In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins and ex vivo systems via biochemical and electrophysiological methods. With the emergence of single-molecule (SM) fluorescence microscopy techniques, it is now possible to characterize the molecular organization and the biophysical dynamics of ion channels in cells with precisions on the order of a f

道学气 发表于 2025-3-25 03:03:56

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查看完整版本: Titlebook: Single Molecule Microscopy in Neurobiology; Nobuhiko Yamamoto,Yasushi Okada Book 2020 Springer Science+Business Media, LLC, part of Spring